Rat Ras Related C3 Botulinum Toxin SubstRat e 1 (Rac1) ELISA Kit
The Rat RAC1 (Ras-related C3 botulinum toxin substrate 1) ELISA Kit is a powerful tool for the quantitative measurement of RAC1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, offering reliable and accurate results for a variety of research applications.RAC1 is a key protein involved in cellular signaling pathways, regulating processes such as cell migration, cell adhesion, and cell cycle progression.
Dysregulation of RAC1 activity has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions. Therefore, the Rat RAC1 ELISA Kit is an indispensable tool for studying RAC1 signaling and its implications in disease pathology, as well as for identifying potential therapeutic targets.
Product Name:
Rat Ras Related C3 Botulinum Toxin SubstRat e 1 (Rac1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
83-98
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
