Rat RegeneRat ing Islet Derived Protein 3 Alpha (REG3a) ELISA Kit
The Rat Regenerating Islet-Derived Protein 3 Alpha (REG3A) ELISA Kit is specifically designed for the precise measurement of REG3A levels in rat serum, plasma, and tissue homogenates. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.REG3A is a vital protein known for its role in promoting tissue regeneration and modulating immune responses. It plays a crucial part in various physiological processes and is linked to inflammatory diseases, diabetes, and tissue repair mechanisms.
The REG3A ELISA Kit is a valuable tool for studying these conditions and developing potential therapeutic interventions.Overall, the Rat Regenerating Islet-Derived Protein 3 Alpha (REG3A) ELISA Kit offers researchers a reliable and efficient method for quantifying REG3A levels in rat samples, enabling a deeper understanding of its biological functions and its implications in disease pathology.
Product Name:
Rat RegeneRat ing Islet Derived Protein 3 Alpha (REG3a) ELISA Kit
Product Code:
RTDL00900
Size:
96 Assays
Target:
REG3a
Synonyms:
HIP, INGAP, PAP-H, PAP1, PBCGF, REG-III, REG3, Pancreatitis-Associated Protein, Hepatointestinal pancreatic protein, Human proislet peptide, Pancreatitis-associated protein 1
Detection Method:
Competition
Range:
6.25-400pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
