The Rat Replication Protein A1 (RPA1) ELISA Kit is a highly reliable and accurate assay designed for the detection of RPA1 levels in rat serum, plasma, and tissue homogenates. This kit offers superior sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.RPA1 is a key protein involved in DNA replication and repair processes, playing a critical role in maintaining genomic stability. Dysregulation of RPA1 has been linked to various diseases, including cancer and genetic disorders.
Studying RPA1 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Using the Rat RPA1 ELISA Kit, researchers can confidently measure RPA1 expression levels in rat samples, enabling in-depth investigation of its biological functions and implications in disease pathogenesis. This kit is a valuable tool for advancing scientific knowledge and driving innovative research in the fields of molecular biology and genetics.
Product Name:
Rat Replication Protein A1 (RPA1) ELISA Kit
Product Code:
RTDL00915
Size:
96 Assays
Target:
RPA1
Synonyms:
HSSB, REPA1, RF-A, RP-A, RPA70, Replication protein A 70 kDa DNA-binding subunit, Single-stranded DNA-binding protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
