The Rat Retn (Resistin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of resistin levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for various research applications.Resistin is a key adipokine involved in inflammation, insulin resistance, and metabolic disorders. Elevated levels of resistin have been associated with obesity, type 2 diabetes, and cardiovascular diseases, highlighting its importance as a potential biomarker for studying these conditions and developing new therapies.
Overall, the Rat Retn (Resistin) CLIA Kit is a valuable tool for researchers studying metabolic disorders and seeking to understand the role of resistin in various physiological processes.
Product Name:
Rat RETN (Resistin) CLIA Kit
SKU:
RTES00379
Target:
Rat RETN (Resistin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat RETN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RETN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat RETN. You can calculate the concentration of Rat RETN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
89-99
98-113
Average (%)
98
94
104
1:4
Range (%)
85-97
91-104
103-118
Average (%)
91
98
110
1:8
Range (%)
97-111
85-97
88-103
Average (%)
102
92
95
1:16
Range (%)
104-118
90-103
97-110
Average (%)
110
97
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
94-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
186.69
602.24
1922.56
174.11
626.93
1939.57
Standard deviation
23.6
65.34
131.7
14.21
49.46
169.91
C V (%)
12.64
10.85
6.85
8.16
7.89
8.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat RETN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat RETN in samples. No significant cross-reactivity or interference between Rat RETN and analogues was observed.
