Rat Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) ELISA Kit
The Rat Rho-Associated Coiled-Coil Containing Protein Kinase 2 (ROCK2) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ROCK2 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it ideal for various research applications.ROCK2 is a key enzyme involved in regulating cell morphology, motility, and cytokinesis. It plays a crucial role in cell migration, proliferation, and apoptosis, making it a valuable biomarker for studying various cellular processes and disease pathologies.
With its high-quality components and user-friendly protocol, the Rat ROCK2 ELISA Kit offers researchers a powerful tool for investigating the role of ROCK2 in various physiological and pathological conditions, including cancer, cardiovascular diseases, and inflammatory disorders.
Product Name:
Rat Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) ELISA Kit
Product Code:
RTDL00914
Size:
96 Assays
Target:
Rock2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
89-99
94
Heparin plasma (n=5)
90-98
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
