The Rat RTN4 (Reticulon 4) CLIA Kit is a highly reliable and accurate assay designed for the quantitative detection of RTN4 levels in rat samples. With its high sensitivity and specificity, this kit provides researchers with dependable results for a variety of research applications.RTN4, also known as Nogo-A, is a key protein involved in the regulation of neuronal growth and regeneration. Dysregulation of RTN4 has been linked to conditions such as spinal cord injury, multiple sclerosis, and Alzheimer's disease, making it a valuable biomarker for studying these diseases and developing potential treatments.
Overall, the Rat RTN4 CLIA Kit offers researchers a powerful tool for investigating the role of RTN4 in neurological disorders and advancing our understanding of neuroregeneration mechanisms.
Product Name:
Rat RTN4 (Reticulon 4) CLIA Kit
SKU:
RTES00634
Target:
Rat RTN4 (Reticulon 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat RTN4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RTN4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RTN4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat RTN4. You can calculate the concentration of Rat RTN4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
99-114
87-100
Average (%)
98
105
93
1:4
Range (%)
97-110
100-115
85-98
Average (%)
105
109
92
1:8
Range (%)
89-103
96-110
85-96
Average (%)
95
103
91
1:16
Range (%)
96-110
89-104
94-107
Average (%)
104
96
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
98-113
106
Cell culture media (n=5)
88-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
219.68
420.0
1974.59
205.26
396.76
1845.96
Standard deviation
23.9
36.25
152.04
18.14
34.64
163.0
C V (%)
10.88
8.63
7.7
8.84
8.73
8.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat RTN4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat RTN4 in samples. No significant cross-reactivity or interference between Rat RTN4 and analogues was observed.
