Rat RUNX2 (Runt Related Transcription Factor 2) CLIA Kit
The Rat RUNX2 (Runt-related transcription factor 2) CLIA Kit is specifically designed for the quantification of RUNX2 levels in rat samples. This kit offers superior sensitivity and specificity for accurate and reproducible results, making it a valuable tool for researchers working in the fields of skeletal development, bone formation, and osteogenesis.RUNX2 is a key transcription factor involved in the regulation of osteoblast differentiation and bone formation.
Aberrant RUNX2 expression has been linked to various skeletal disorders, making it a crucial biomarker for understanding bone metabolism and pathologies.With the Rat RUNX2 CLIA Kit, researchers can reliably measure levels of this important transcription factor in serum, plasma, and tissue samples, providing valuable insights into the molecular mechanisms underlying skeletal health and disease. Upgrade your research with the Rat RUNX2 CLIA Kit from Assay Genie.
Product Name:
Rat RUNX2 (Runt Related Transcription Factor 2) CLIA Kit
SKU:
RTES00503
Target:
Rat RUNX2 (Runt Related Transcription Factor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat RUNX2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RUNX2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RUNX2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat RUNX2. You can calculate the concentration of Rat RUNX2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
87-103
97-111
Average (%)
100
94
104
1:4
Range (%)
99-112
97-109
99-115
Average (%)
105
103
106
1:8
Range (%)
97-113
93-106
92-104
Average (%)
104
99
98
1:16
Range (%)
90-103
84-99
94-110
Average (%)
97
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
104
EDTA plasma (n=5)
92-105
100
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.14
626.83
1569.76
187.48
569.38
1437.64
Standard deviation
19.16
51.15
106.43
22.89
47.03
98.62
C V (%)
9.97
8.16
6.78
12.21
8.26
6.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat RUNX2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat RUNX2 in samples. No significant cross-reactivity or interference between Rat RUNX2 and analogues was observed.
