Rat S100A6 (S100 Calcium-Binding Protein A6) CLIA Kit
The Rat S100A6 (S100 Calcium Binding Protein A6) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of S100A6 levels in rat samples. This kit is perfect for researchers studying the role of S100A6 in various biological processes, including cell proliferation, differentiation, and calcium signal transduction.S100A6 is a member of the S100 protein family, known for its calcium-binding properties and involvement in various cellular processes. It plays a crucial role in inflammation, cellular stress responses, and cancer progression, making it a valuable biomarker for understanding disease mechanisms and developing potential therapeutic interventions.
With its advanced technology and user-friendly format, the Rat S100A6 CLIA Kit provides reliable and reproducible results, ensuring the success of your research projects. Don't miss out on this essential tool for investigating the functions of S100A6 in rat models. Order your kit today and take your research to the next level.
Product Name:
Rat S100A6 (S100 Calcium-Binding Protein A6) CLIA Kit
SKU:
RTES00506
Target:
Rat S100A6 (S100 Calcium-Binding Protein A6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat S100A6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat S100A6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat S100A6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat S100A6. You can calculate the concentration of Rat S100A6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
88-100
102-118
Average (%)
104
95
109
1:4
Range (%)
102-116
86-98
94-110
Average (%)
108
91
101
1:8
Range (%)
86-97
97-114
103-119
Average (%)
91
105
109
1:16
Range (%)
85-97
100-114
92-105
Average (%)
90
105
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
86-102
93
Cell culture media (n=5)
85-99
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.02
214.87
993.61
110.55
197.23
1057.19
Standard deviation
10.04
23.51
103.04
11.92
15.42
81.09
C V (%)
9.13
10.94
10.37
10.78
7.82
7.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat S100A6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat S100A6 in samples. No significant cross-reactivity or interference between Rat S100A6 and analogues was observed.
