The Rat SAA (Serum Amyloid A) CLIA Kit is a reliable and effective tool for quantifying SAA levels in rat serum samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for research applications.Serum Amyloid A (SAA) is an important acute-phase protein that is elevated in response to inflammation, infection, and tissue injury. Elevated SAA levels have been associated with various inflammatory conditions, making it a valuable biomarker for studying and monitoring disease progression.
With the Rat SAA CLIA Kit, researchers can accurately measure SAA levels in rat samples, providing valuable insights into the inflammatory response and disease pathology. This kit is easy to use and provides fast results, making it an essential tool for studying inflammatory diseases in rat models.
Product Name:
Rat SAA (Serum Amyloid A) CLIA Kit
SKU:
RTES00513
Target:
Rat SAA (Serum Amyloid A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SAA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SAA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SAA. You can calculate the concentration of Rat SAA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
85-100
85-98
Average (%)
109
92
92
1:4
Range (%)
95-105
97-109
98-112
Average (%)
100
104
104
1:8
Range (%)
93-109
89-105
92-104
Average (%)
101
96
97
1:16
Range (%)
97-112
100-117
84-99
Average (%)
102
109
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
102
EDTA plasma (n=5)
99-113
106
Cell culture media (n=5)
89-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.05
283.62
742.8
98.44
281.65
669.95
Standard deviation
12.68
33.92
54.52
11.04
28.17
64.38
C V (%)
12.43
11.96
7.34
11.21
10.0
9.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SAA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SAA in samples. No significant cross-reactivity or interference between Rat SAA and analogues was observed.
