The Rat SAP (Serum Amyloid P Component) ELISA Kit is a specialized assay designed to quantitatively measure the levels of Serum Amyloid P Component in various rat biological samples. Serum Amyloid P Component is a pentameric protein that plays a crucial role in the regulation of inflammation and tissue repair. Its interactions with amyloid fibrils and extracellular matrix components contribute to the modulation of immune responses and tissue homeostasis. This ELISA kit enables researchers to accurately assess the levels of Serum Amyloid P Component, shedding light on its involvement in inflammatory processes, amyloidosis, and other pathological conditions.
By studying the dynamics of Serum Amyloid P Component expression, researchers can gain valuable insights into its role in immune regulation, tissue remodeling, and disease pathogenesis. The Rat SAP ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results in experimental settings. Manufactified under strict quality control standards, this kit delivers reliable performance and ease of use, making it an ideal tool for investigating the functions and therapeutic implications of Serum Amyloid P Component in rat models.
Product Name:
Rat SAP (Serum Amyloid P Component) ELISA Kit
SKU:
AEES00561
Target:
Rat SAP (Serum Amyloid P Component)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SAP. You can calculate the concentration of Rat SAP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
90-104
93-107
Average (%)
98
95
100
1:4
Range (%)
93-108
90-101
92-107
Average (%)
100
95
99
1:8
Range (%)
91-102
90-103
95-110
Average (%)
96
96
101
1:16
Range (%)
94-107
92-105
90-102
Average (%)
101
98
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.2
218.76
702.05
98.45
239.37
760.25
Standard deviation
6.58
12.93
30.12
6.1
13.19
27.22
C V (%)
6.25
5.91
4.29
6.2
5.51
3.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SAP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SAP in samples. No significant cross-reactivity or interference between Rat SAP and analogues was observed.
