The Rat SCF (Stem Cell Factor) CLIA Kit is a powerful tool for researchers looking to accurately measure levels of SCF in rat samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.SCF is a key cytokine that plays a critical role in the regulation of hematopoiesis, stem cell maintenance, and cell proliferation. Dysregulation of SCF has been linked to various diseases, including cancer and inflammatory disorders, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions.
With the Rat SCF CLIA Kit, researchers can gain valuable insights into the role of SCF in health and disease, paving the way for new discoveries and advancements in the field of stem cell biology and regenerative medicine.
Product Name:
Rat SCF (Stem Cell Factor) CLIA Kit
SKU:
RTES00526
Target:
Rat SCF (Stem Cell Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SCF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SCF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SCF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SCF. You can calculate the concentration of Rat SCF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
98-115
94-107
Average (%)
106
105
102
1:4
Range (%)
99-115
99-117
95-109
Average (%)
105
107
103
1:8
Range (%)
96-112
84-98
101-111
Average (%)
102
91
106
1:16
Range (%)
92-104
98-114
87-99
Average (%)
97
106
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
104
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
99-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.61
69.24
167.83
24.72
64.8
161.14
Standard deviation
3.43
5.03
16.25
2.7
4.7
18.31
C V (%)
12.89
7.26
9.68
10.92
7.25
11.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SCF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SCF in samples. No significant cross-reactivity or interference between Rat SCF and analogues was observed.
