The Rat SDC1 (Syndecan-1) ELISA Kit is specifically designed for the accurate measurement of Syndecan-1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Syndecan-1 is a cell surface proteoglycan that plays a critical role in cell adhesion, migration, and signaling. It is involved in various physiological and pathological processes, including inflammation, wound healing, and cancer progression.
Detection of Syndecan-1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its easy-to-use format and robust performance, the Rat SDC1 ELISA Kit is an essential tool for researchers studying Syndecan-1 biology and its implications in health and disease. Get accurate and reproducible results with this comprehensive kit from Assay Genie.
Product Name:
Rat SDC1 (Syndecan 1) ELISA Kit
SKU:
RTES00832
Target:
Rat SDC1 (Syndecan 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SDC1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SDC1. You can calculate the concentration of Rat SDC1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
94-109
91-107
Average (%)
99
102
98
1:4
Range (%)
90-104
84-95
86-100
Average (%)
96
89
91
1:8
Range (%)
92-106
81-92
82-93
Average (%)
98
86
88
1:16
Range (%)
89-101
85-97
81-93
Average (%)
96
92
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.89
14.44
42.98
5.15
14.33
44.03
Standard deviation
0.26
0.6
1.82
0.32
0.8
1.39
C V (%)
5.32
4.16
4.23
6.21
5.58
3.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SDC1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SDC1 in samples. No significant cross-reactivity or interference between Rat SDC1 and analogues was observed.
