The Rat Sele (E-Selectin) CLIA Kit is specifically designed for the precise measurement of E-selectin levels in rat serum, plasma, and cell culture samples. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.E-selectin is a key molecule involved in the inflammatory response, playing a crucial role in the adhesion and recruitment of leukocytes to sites of inflammation.
It serves as a valuable biomarker for studying inflammatory conditions, such as autoimmune diseases, atherosclerosis, and sepsis, and for developing targeted therapies to treat these ailments.Overall, the Rat Sele (E-Selectin) CLIA Kit is a valuable tool for researchers seeking to investigate the role of E-selectin in various disease states and to potentially discover new treatment options.
Product Name:
Rat SELE (E-Selectin) CLIA Kit
SKU:
RTES00517
Target:
Rat SELE (E-Selectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SELE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SELE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SELE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SELE. You can calculate the concentration of Rat SELE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
99-112
92-105
Average (%)
94
105
98
1:4
Range (%)
102-114
99-116
93-106
Average (%)
108
107
99
1:8
Range (%)
99-115
97-109
87-97
Average (%)
106
103
92
1:16
Range (%)
98-117
85-99
85-100
Average (%)
107
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.02
91.82
424.17
44.89
89.53
397.69
Standard deviation
4.33
6.45
43.01
4.31
6.28
37.34
C V (%)
9.02
7.02
10.14
9.6
7.01
9.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SELE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SELE in samples. No significant cross-reactivity or interference between Rat SELE and analogues was observed.
