The Rat L-Selectin CLIA Kit is specifically designed for the precise measurement of L-Selectin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research purposes.L-Selectin is a key cell adhesion molecule that plays a critical role in mediating leukocyte adhesion to endothelial cells during inflammation.
Dysregulation of L-Selectin has been linked to various inflammatory conditions, making it a valuable biomarker for studying immune responses and developing therapeutic interventions.With its user-friendly protocol and reliable performance, the Rat L-Selectin CLIA Kit is a must-have for researchers looking to explore the role of L-Selectin in inflammatory diseases and immune system function in rat models.
Product Name:
Rat SELL (L-Selectin) CLIA Kit
SKU:
RTES00366
Target:
Rat SELL (L-Selectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SELL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SELL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SELL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SELL. You can calculate the concentration of Rat SELL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
95-105
101-116
Average (%)
99
100
108
1:4
Range (%)
91-104
85-96
92-107
Average (%)
96
90
98
1:8
Range (%)
98-112
98-111
89-106
Average (%)
105
104
97
1:16
Range (%)
102-119
101-115
103-116
Average (%)
110
106
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
92
EDTA plasma (n=5)
92-108
98
Cell culture media (n=5)
90-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.7
202.04
907.56
87.65
185.59
944.58
Standard deviation
11.62
21.92
61.26
11.03
21.6
105.32
C V (%)
12.14
10.85
6.75
12.58
11.64
11.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SELL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SELL in samples. No significant cross-reactivity or interference between Rat SELL and analogues was observed.
