The Rat SELP (P-Selectin) ELISA Kit is a cutting-edge assay meticulously developed for the quantitative assessment of P-Selectin levels in diverse rat biological samples. P-Selectin, a prominent cell adhesion molecule, is instrumental in mediating inflammatory responses and facilitating interactions between endothelial cells and leukocytes. With a pivotal role in regulating leukocyte trafficking and recruitment to sites of inflammation, P-Selectin serves as a critical mediator in various immunological processes and vascular responses. This ELISA kit offers researchers a precise and reliable method to quantify P-Selectin expression levels, providing invaluable insights into its involvement in immune cell adhesion and inflammation.
By accurately measuring P-Selectin, researchers can enhance their understanding of the intricate mechanisms governing leukocyte recruitment and endothelial activation, shedding light on the pathophysiology of inflammatory conditions and vascular disorders. Crafted with exceptional sensitivity and specificity, the Rat SELP ELISA Kit ensures accurate and reproducible results, enabling comprehensive analyses of P-Selectin dynamics in biological samples. With stringent quality control measures in place, this kit delivers robust and consistent performance, offering researchers a user-friendly and efficient tool for exploring the intricate roles of P-Selectin in immune responses and inflammatory pathways.
Product Name:
Rat SELP (P-Selectin) ELISA Kit
SKU:
AEES00554
Target:
Rat SELP (P-Selectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SELP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SELP. You can calculate the concentration of Rat SELP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
92-105
85-96
Average (%)
104
97
91
1:4
Range (%)
93-109
89-104
84-99
Average (%)
100
96
91
1:8
Range (%)
97-114
92-105
80-94
Average (%)
104
98
87
1:16
Range (%)
94-106
92-105
80-91
Average (%)
100
99
86
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
272.98
776.41
1905.72
261.66
715.67
1816.89
Standard deviation
14.9
43.87
89.38
16.96
30.99
89.21
C V (%)
5.46
5.65
4.69
6.48
4.33
4.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SELP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SELP in samples. No significant cross-reactivity or interference between Rat SELP and analogues was observed.
