The Rat SEMA7A (Semaphorin 7A) CLIA Kit is a reliable and accurate tool for detecting SEMA7A levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reproducible results for a variety of research applications.SEMA7A is a key protein involved in cell signaling and immune response regulation. It plays a crucial role in processes such as cell migration, axon guidance, and immune cell activation.
Dysregulation of SEMA7A is associated with various diseases, including cancer, inflammatory disorders, and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Rat SEMA7A CLIA Kit is a powerful tool for researchers looking to investigate the role of SEMA7A in health and disease, providing accurate and reliable data for further exploration and discovery.
Product Name:
Rat SEMA7A (Semaphorin 7A) CLIA Kit
SKU:
RTES00512
Target:
Rat SEMA7A (Semaphorin 7A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SEMA7A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SEMA7A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SEMA7A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SEMA7A. You can calculate the concentration of Rat SEMA7A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
87-98
102-116
Average (%)
101
93
110
1:4
Range (%)
86-101
93-106
92-105
Average (%)
93
99
97
1:8
Range (%)
88-100
87-100
85-100
Average (%)
93
92
92
1:16
Range (%)
88-100
95-110
83-96
Average (%)
93
102
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
104
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
200.15
638.42
1783.64
194.63
665.09
1936.07
Standard deviation
25.28
64.99
201.91
24.52
55.67
168.05
C V (%)
12.63
10.18
11.32
12.6
8.37
8.68
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SEMA7A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SEMA7A in samples. No significant cross-reactivity or interference between Rat SEMA7A and analogues was observed.
