The Rat SEPP1 (Selenoprotein P) CLIA Kit is specifically designed for the quantitative measurement of Selenoprotein P levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and accuracy, providing reliable and reproducible results for a variety of research applications.Selenoprotein P is an important protein that plays a critical role in selenium transport and antioxidant defense in the body. It is involved in various biological processes, including immune response, thyroid hormone metabolism, and inflammation.
Abnormal levels of Selenoprotein P have been linked to conditions such as diabetes, cardiovascular diseases, and thyroid disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions. Overall, the Rat SEPP1 CLIA Kit offers researchers a powerful tool for investigating the role of Selenoprotein P in health and disease, providing valuable insights into its potential as a therapeutic target.
Product Name:
Rat SEPP1 (Selenoprotein P) CLIA Kit
SKU:
RTES00511
Target:
Rat SEPP1 (Selenoprotein P)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SEPP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SEPP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SEPP1. You can calculate the concentration of Rat SEPP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
97-109
87-99
Average (%)
105
102
93
1:4
Range (%)
89-102
99-113
100-112
Average (%)
96
105
106
1:8
Range (%)
92-105
95-111
92-105
Average (%)
98
103
100
1:16
Range (%)
103-119
94-109
98-112
Average (%)
110
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
84-97
90
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.94
243.48
697.14
92.31
219.58
762.37
Standard deviation
12.45
18.77
76.34
8.78
15.66
84.47
C V (%)
12.98
7.71
10.95
9.51
7.13
11.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SEPP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SEPP1 in samples. No significant cross-reactivity or interference between Rat SEPP1 and analogues was observed.
