Rat sFAS/sAPO-1 (Soluble Factor-Related Apoptosis) CLIA Kit
The Rat SFAS (Sapo-1 Soluble Factor-Related Apoptosis) CLIA Kit from Assay Genie is a highly sensitive and specific assay designed for the accurate detection of SFAS levels in rat samples. This kit is ideal for researchers studying apoptosis-related pathways and examining the role of SFAS in various biological processes.SFAS is a key factor in apoptosis, or programmed cell death, and plays a crucial role in regulating cell survival and death. Dysregulation of SFAS has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions, making it a valuable biomarker for research and potential therapeutic targets.
With its reliable performance and user-friendly protocol, the Rat SFAS CLIA Kit provides researchers with a valuable tool for investigating the molecular mechanisms underlying apoptosis and identifying novel therapeutic strategies. Discover the potential of SFAS with the Assay Genie Rat SFAS CLIA Kit today.
Product Name:
Rat sFAS/sAPO-1 (Soluble Factor-Related Apoptosis) CLIA Kit
SKU:
RTES00518
Target:
Rat sFAS/sAPO-1 (Soluble Factor-Related Apoptosis)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat sFAS/sAPO-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sFAS/sAPO-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sFAS/sAPO-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat sFAS/sAPO-1. You can calculate the concentration of Rat sFAS/sAPO-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
84-96
95-107
Average (%)
103
90
101
1:4
Range (%)
102-115
100-111
100-116
Average (%)
108
105
107
1:8
Range (%)
87-101
89-99
97-108
Average (%)
94
94
102
1:16
Range (%)
91-103
83-97
96-108
Average (%)
97
90
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-114
105
EDTA plasma (n=5)
97-108
103
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.05
129.68
427.47
49.82
126.07
439.35
Standard deviation
5.12
11.79
48.3
4.19
10.02
35.46
C V (%)
10.66
9.09
11.3
8.41
7.95
8.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat sFAS/sAPO-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat sFAS/sAPO-1 in samples. No significant cross-reactivity or interference between Rat sFAS/sAPO-1 and analogues was observed.
