Rat Signal Transducer And Activator Of Transcription 6 (STAT6) ELISA Kit
The Rat Signal Transducer and Activator of Transcription 6 (STAT6) ELISA Kit is a powerful tool for the precise quantification of STAT6 levels in rat serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers reproducible and accurate results, making it indispensable for a wide array of research studies.STAT6 is a key signaling protein that plays a critical role in immune responses and inflammatory processes. Its dysregulation has been linked to various diseases, including asthma, allergies, and autoimmune disorders.
By measuring STAT6 levels, researchers can gain valuable insights into the molecular mechanisms underlying these conditions and potentially uncover new therapeutic targets.Don't miss out on the opportunity to advance your research with the Rat STAT6 ELISA Kit - the ultimate solution for studying the intricate functions of STAT6 in rats and advancing scientific knowledge in immunology and beyond.
Product Name:
Rat Signal Transducer And Activator Of Transcription 6 (STAT6) ELISA Kit
Product Code:
RTDL00998
Size:
96 Assays
Target:
STAT6
Synonyms:
IL-4-STAT, STAT6B, STAT6C, Signal Transducer And Activator Of Transcription 6,Interleukin-4 Induced
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
