Rat SLC (Secondary Lymphoid Tissue Chemokine) CLIA Kit
The Rat SLC (Secondary Lymphoid Tissue Chemokine) CLIA Kit is specifically designed for the quantitative measurement of SLC levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and accuracy, ensuring dependable and consistent results for various research applications.SLC is a chemokine that plays a critical role in immune responses, specifically in the homing of lymphocytes to secondary lymphoid organs.
Its dysregulation has been associated with various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Rat SLC CLIA Kit from Assay Genie provides researchers with a reliable tool for investigating the role of SLC in immune responses and its potential implications in disease pathogenesis.
Product Name:
Rat SLC (Secondary Lymphoid Tissue Chemokine) CLIA Kit
SKU:
RTES00390
Target:
Rat SLC (Secondary Lymphoid Tissue Chemokine)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SLC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SLC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SLC. You can calculate the concentration of Rat SLC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
84-96
99-114
Average (%)
95
91
105
1:4
Range (%)
94-110
99-112
92-103
Average (%)
100
105
98
1:8
Range (%)
91-107
89-102
98-110
Average (%)
97
95
105
1:16
Range (%)
87-103
94-108
99-113
Average (%)
94
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
85-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.19
132.84
353.51
48.15
145.94
356.32
Standard deviation
5.6
14.76
28.14
5.6
15.34
29.43
C V (%)
10.73
11.11
7.96
11.63
10.51
8.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SLC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SLC in samples. No significant cross-reactivity or interference between Rat SLC and analogues was observed.
