Rat SNAP-25 (Synaptosome Associated Protein 25) CLIA Kit
The Rat SNAP-25 (Synaptosome-Associated Protein 25) CLIA Kit is specifically designed for the quantitative detection of SNAP-25 levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for research applications in neuroscience and neurodegenerative diseases.SNAP-25 is a critical protein involved in synaptic vesicle exocytosis and neurotransmitter release, essential for proper neuronal function and communication. Dysregulation of SNAP-25 has been implicated in various neurological disorders, including epilepsy, schizophrenia, and Alzheimer's disease.
Therefore, measuring SNAP-25 levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of targeted therapies.With easy-to-follow protocols and rapid assay times, the Rat SNAP-25 CLIA Kit from Assay Genie is a valuable tool for researchers looking to study synaptic function and explore potential drug targets for neurological disorders. Order your kit today and unlock new possibilities in neuroscience research.
Product Name:
Rat SNAP-25 (Synaptosome Associated Protein 25) CLIA Kit
SKU:
RTES00531
Target:
Rat SNAP-25 (Synaptosome Associated Protein 25)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SNAP-25. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SNAP-25 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SNAP-25, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SNAP-25. You can calculate the concentration of Rat SNAP-25 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
94-108
100-116
Average (%)
92
101
106
1:4
Range (%)
98-113
88-101
87-99
Average (%)
106
93
94
1:8
Range (%)
97-112
94-106
97-112
Average (%)
104
100
103
1:16
Range (%)
92-106
85-98
87-101
Average (%)
97
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
92
EDTA plasma (n=5)
98-114
106
Cell culture media (n=5)
100-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.86
210.84
974.52
100.78
193.61
949.85
Standard deviation
14.06
21.95
98.91
8.91
18.41
59.75
C V (%)
12.68
10.41
10.15
8.84
9.51
6.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SNAP-25 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SNAP-25 in samples. No significant cross-reactivity or interference between Rat SNAP-25 and analogues was observed.
