The Rat SNCA (Synuclein Alpha) ELISA Kit is specifically developed for the precise measurement of synuclein alpha levels in rat serum, plasma, and cell culture supernatants. With outstanding sensitivity and specificity, this kit delivers consistent and dependable results, making it perfect for various research purposes.Synuclein alpha is a key protein associated with neurodegenerative diseases, particularly Parkinson's disease.
Its role in protein aggregation and neuronal cell damage makes it a valuable biomarker for studying neurodegenerative disorders and exploring potential treatment options.Overall, the Rat SNCA (Synuclein Alpha) ELISA Kit is a valuable tool for researchers investigating the mechanisms of neurodegenerative diseases and seeking novel therapeutic approaches.
Product Name:
Rat SNCa (Synuclein Alpha) ELISA Kit
SKU:
RTES00961
Target:
Rat SNCa (Synuclein Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SNCα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SNCα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SNCα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SNCα. You can calculate the concentration of Rat SNCα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
92-106
90-106
Average (%)
104
99
97
1:4
Range (%)
90-100
80-91
85-97
Average (%)
95
86
92
1:8
Range (%)
88-100
84-95
81-94
Average (%)
95
89
88
1:16
Range (%)
92-107
81-92
83-95
Average (%)
98
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.79
112.15
482.01
50.05
122.68
440.06
Standard deviation
2.49
6.15
20.53
2.56
5.02
22.14
C V (%)
5.44
5.48
4.26
5.11
4.09
5.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SNCα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SNCα in samples. No significant cross-reactivity or interference between Rat SNCα and analogues was observed.
