Rat SOCS3 (Suppressors Of Cytokine Signaling 3) CLIA Kit
The Rat SOCS3 (Suppressors of Cytokine Signaling 3) CLIA Kit is specifically designed for the accurate detection of SOCS3 levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.SOCS3 is a key regulator of cytokine signaling pathways, playing a critical role in immune response and inflammation. Dysregulation of SOCS3 has been linked to various diseases such as cancer, autoimmune disorders, and metabolic diseases.
Therefore, accurate measurement of SOCS3 levels is essential for understanding disease mechanisms and potential therapeutic interventions.With its reliable performance and user-friendly design, the Rat SOCS3 CLIA Kit is a valuable tool for researchers studying cytokine signaling, inflammation, and related diseases in rat models. Trust in this kit to deliver precise and reliable results for your research needs.
Product Name:
Rat SOCS3 (Suppressors Of Cytokine Signaling 3) CLIA Kit
SKU:
RTES00529
Target:
Rat SOCS3 (Suppressors Of Cytokine Signaling 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SOCS3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SOCS3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SOCS3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SOCS3. You can calculate the concentration of Rat SOCS3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
95-110
91-105
Average (%)
102
101
97
1:4
Range (%)
103-118
97-109
87-99
Average (%)
109
104
93
1:8
Range (%)
99-113
86-99
92-109
Average (%)
105
91
100
1:16
Range (%)
89-102
93-108
90-102
Average (%)
94
99
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
106
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
97-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.54
171.69
769.41
93.25
155.39
833.75
Standard deviation
9.73
12.12
50.32
8.35
17.26
69.53
C V (%)
10.63
7.06
6.54
8.95
11.11
8.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SOCS3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SOCS3 in samples. No significant cross-reactivity or interference between Rat SOCS3 and analogues was observed.
