Rat SOD1 ( Superoxide Dismutase 1, Soluble) ELISA Kit
The Rat SOD1 (Superoxide Dismutase 1) Soluble ELISA Kit is specifically designed for the accurate quantification of soluble SOD1 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.SOD1 is a critical enzyme that plays a key role in antioxidant defense by catalyzing the dismutation of superoxide radicals. Dysregulation of SOD1 has been implicated in various diseases including neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions.
This ELISA kit provides a valuable tool for studying the role of SOD1 in these diseases and developing potential therapeutic interventions.Overall, the Rat SOD1 Soluble ELISA Kit is a reliable and efficient tool for researchers looking to investigate the involvement of SOD1 in various physiological and pathological processes.
Product Name:
Rat SOD1 ( Superoxide Dismutase 1, Soluble) ELISA Kit
SKU:
RTES00994
Target:
Rat SOD1 ( Superoxide Dismutase 1, Soluble)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD1. During the reaction, SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
90-104
92-105
Average (%)
98
96
100
1:4
Range (%)
84-97
91-103
100-115
Average (%)
90
98
107
1:8
Range (%)
87-99
94-104
95-107
Average (%)
92
99
101
1:16
Range (%)
86-98
89-102
96-110
Average (%)
93
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
89-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.2
3.4
0.5
1.3
3.6
Standard deviation
0.03
0.07
0.12
0.03
0.07
0.13
C V (%)
6
5.83
3.53
6.0
5.38
3.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of SOD1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed.
