Rat SP-A (Pulmonary Surfatcant-Associated Protein A) CLIA Kit
The Rat SP-A (Pulmonary Surfactant Associated Protein A) CLIA Kit is a highly specific and sensitive assay designed for the accurate measurement of SP-A levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an ideal tool for researchers studying pulmonary surfactant proteins and their role in respiratory health.SP-A is a vital component of the lung surfactant system, playing a crucial role in maintaining lung function and protecting against respiratory infections. Changes in SP-A levels have been associated with various lung diseases such as pulmonary fibrosis, pneumonia, and acute respiratory distress syndrome (ARDS), highlighting the importance of studying this protein in the context of respiratory health.
By using the Rat SP-A CLIA Kit, researchers can accurately quantify SP-A levels in rat samples, providing valuable insights into the mechanisms underlying lung diseases and potential therapeutic interventions. The kit's high sensitivity and specificity ensure accurate and reliable results, making it a valuable tool for advancing research in respiratory biology.
Product Name:
Rat SP-A (Pulmonary Surfatcant-Associated Protein A) CLIA Kit
SKU:
RTES00038
Target:
Rat SP-A (Pulmonary Surfatcant-Associated Protein A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SP-A1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SP-A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SP-A1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SP-A1. You can calculate the concentration of Rat SP-A1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
101-114
93-111
Average (%)
98
108
101
1:4
Range (%)
95-107
88-102
86-99
Average (%)
101
94
93
1:8
Range (%)
84-100
86-98
97-110
Average (%)
91
93
104
1:16
Range (%)
101-114
88-103
97-109
Average (%)
108
95
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
104
EDTA plasma (n=5)
96-111
103
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.83
119.1
317.22
38.48
112.9
345.99
Standard deviation
4.65
11.77
28.39
4.24
11.93
37.96
C V (%)
11.39
9.88
8.95
11.02
10.57
10.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SP-A1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SP-A1 in samples. No significant cross-reactivity or interference between Rat SP-A1 and analogues was observed.
