Rat SP-B (Pulmonary Surfatcant-Associated Protein B) CLIA Kit
The Rat SP-B (Pulmonary Surfactant-Associated Protein B) CLIA Kit is a cutting-edge assay designed for the precise measurement of SP-B levels in rat samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research applications.SP-B, a vital component of pulmonary surfactant, plays a crucial role in maintaining lung function and preventing respiratory distress syndrome in neonates. Dysregulation of SP-B levels has been linked to various lung diseases, making it a valuable biomarker for studying these conditions and exploring potential treatment options.
With its advanced technology and reliable performance, the Rat SP-B CLIA Kit is an indispensable tool for researchers in the fields of respiratory medicine, toxicology, and drug development. Trust in this innovative kit to facilitate your research and drive new discoveries in the field of pulmonary biology.
Product Name:
Rat SP-B (Pulmonary Surfatcant-Associated Protein B) CLIA Kit
SKU:
RTES00049
Target:
Rat SP-B (Pulmonary Surfatcant-Associated Protein B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SP-B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SP-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SP-B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SP-B. You can calculate the concentration of Rat SP-B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
93-108
90-104
Average (%)
90
101
96
1:4
Range (%)
91-102
85-97
93-105
Average (%)
96
91
99
1:8
Range (%)
95-106
85-96
102-116
Average (%)
100
91
109
1:16
Range (%)
92-106
101-114
88-102
Average (%)
98
107
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
104
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
101-119
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
2.24
8.74
1.1
2.44
8.87
Standard deviation
0.11
0.21
0.64
0.11
0.17
0.58
C V (%)
10.28
9.38
7.32
10.0
6.97
6.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SP-B concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SP-B in samples. No significant cross-reactivity or interference between Rat SP-B and analogues was observed.
