Rat SPC (Pulmonary Surfactant Associated Protein C) CLIA Kit
The Rat SPC (Pulmonary Surfactant-Associated Protein C) CLIA Kit is a powerful tool for the precise measurement of SPC levels in rat samples. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, perfect for various research purposes.SPC is a vital protein closely associated with pulmonary function, particularly in maintaining the stability of surfactants in the lungs.
Changes in SPC levels are linked to respiratory diseases, making it a key biomarker in studying these conditions and developing therapeutic interventions.With the Rat SPC CLIA Kit, researchers can confidently analyze and monitor SPC levels in rat samples, paving the way for a deeper understanding of pulmonary health and potential treatment options.
Product Name:
Rat SPC (Pulmonary Surfactant Associated Protein C) CLIA Kit
SKU:
RTES00491
Target:
Rat SPC (Pulmonary Surfactant Associated Protein C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SPC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SPC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SPC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SPC. You can calculate the concentration of Rat SPC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
100-112
101-119
Average (%)
96
106
109
1:4
Range (%)
94-106
95-109
89-99
Average (%)
101
102
94
1:8
Range (%)
91-101
101-116
87-101
Average (%)
96
107
92
1:16
Range (%)
89-103
102-119
88-102
Average (%)
97
109
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
99-114
106
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
428.69
846.11
2777.41
432.0
804.42
2789.86
Standard deviation
51.14
94.43
280.8
46.18
66.04
177.16
C V (%)
11.93
11.16
10.11
10.69
8.21
6.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SPC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SPC in samples. No significant cross-reactivity or interference between Rat SPC and analogues was observed.
