Rat Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA Kit
The Rat Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA Kit is a reliable and accurate tool for quantifying SREBF1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.SREBF1 is a key transcription factor involved in regulating cholesterol and lipid metabolism, playing a critical role in various physiological processes. Dysregulation of SREBF1 is associated with metabolic disorders, cardiovascular diseases, and fatty liver disease, highlighting its importance as a potential therapeutic target and biomarker for disease progression.
By utilizing the Rat SREBF1 ELISA Kit, researchers can explore the role of SREBF1 in different disease states, elucidate its molecular mechanisms, and identify potential therapeutic interventions for related conditions. With its high-performance characteristics, this kit is a valuable asset for advancing scientific discoveries in the field of molecular biology and biomedicine.
Product Name:
Rat Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA Kit
Product Code:
RTDL00992
Size:
96 Assays
Target:
SREBF1
Synonyms:
SREBP1, Sterol Regulatory Element-Binding Protein 1, Adipocyte determination- and differentiation-dependent factor 1, ADD1
Detection Method:
Sandwich
Range:
0.39-25ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
