The Rat SYP (Synaptophysin) CLIA Kit is a highly sensitive assay designed specifically for the accurate detection of synaptophysin levels in rat samples. This kit is ideal for researchers studying synaptic function and neurotransmission in the brain, as synaptophysin is a key protein located in presynaptic vesicles and is widely used as a marker for synaptic density and neuronal connectivity.Synaptophysin plays a crucial role in neurotransmitter release and synaptic plasticity, making it essential for understanding brain function and neurological disorders.
With its high specificity and reproducibility, the Rat SYP CLIA Kit provides dependable results for a variety of research applications in neuroscience and neurology.Researchers can trust in the reliability and accuracy of the Rat SYP CLIA Kit to advance their understanding of synaptic function and explore new therapeutic opportunities for neurodegenerative diseases, psychiatric disorders, and other conditions affecting the central nervous system.
Product Name:
Rat SYP (Synaptophysin) CLIA Kit
SKU:
RTES00530
Target:
Rat SYP (Synaptophysin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SYP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SYP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SYP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SYP. You can calculate the concentration of Rat SYP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
100-115
93-109
Average (%)
98
106
99
1:4
Range (%)
96-111
91-105
94-105
Average (%)
104
96
100
1:8
Range (%)
88-99
96-110
85-96
Average (%)
94
103
91
1:16
Range (%)
91-105
103-117
88-99
Average (%)
96
109
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
422.69
1221.32
2994.17
410.13
1268.11
2816.02
Standard deviation
54.06
111.51
277.26
48.27
116.54
202.47
C V (%)
12.79
9.13
9.26
11.77
9.19
7.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SYP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SYP in samples. No significant cross-reactivity or interference between Rat SYP and analogues was observed.
