The Rat TACR1 (Tachykinin Receptor 1) CLIA Kit is a valuable tool for researchers looking to accurately measure levels of TACR1 in rat samples. Tachykinin Receptor 1 is a key receptor involved in neurogenic inflammation and has been implicated in a variety of physiological and pathological processes.This kit boasts high sensitivity and specificity, ensuring precise and reliable results for a variety of sample types including serum, plasma, and tissue lysates. By accurately quantifying TACR1 levels, researchers can gain valuable insights into the role of this receptor in various diseases and conditions.
Understanding the expression and activity of Tachykinin Receptor 1 is crucial for advancing research in areas such as pain perception, inflammation, and gastrointestinal disorders. The Rat TACR1 CLIA Kit provides a convenient and effective solution for studying the functions and mechanisms of this important receptor in a laboratory setting.
Product Name:
Rat TACR1 (Tachykinin Receptor 1) CLIA Kit
SKU:
RTES00533
Target:
Rat TACR1 (Tachykinin Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TACR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TACR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TACR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TACR1. You can calculate the concentration of Rat TACR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
100-116
98-113
Average (%)
95
107
106
1:4
Range (%)
98-112
89-103
99-116
Average (%)
104
97
108
1:8
Range (%)
94-109
91-106
102-116
Average (%)
100
97
109
1:16
Range (%)
91-106
91-101
92-108
Average (%)
98
96
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
106
EDTA plasma (n=5)
83-95
90
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.21
295.83
708.96
117.3
278.85
662.17
Standard deviation
12.83
26.15
73.38
14.39
19.58
59.53
C V (%)
11.86
8.84
10.35
12.27
7.02
8.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TACR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TACR1 in samples. No significant cross-reactivity or interference between Rat TACR1 and analogues was observed.
