The Rat TACR2 (Tachykinin Receptor 2) CLIA Kit is a highly sensitive assay designed for the precise measurement of TACR2 levels in rat samples, including serum, plasma, and tissue lysates. This kit offers superior specificity and accuracy, ensuring dependable and reproducible results for a variety of research applications.Tachykinin Receptor 2 is a key receptor involved in the regulation of neurotransmitter release and modulation of sensory nerve functions.
It plays a crucial role in various physiological processes, including pain sensation, inflammation, and gastrointestinal motility. Dysregulation of TACR2 has been linked to conditions such as chronic pain, asthma, and gastrointestinal disorders, making it a valuable biomarker for studying these diseases and developing targeted therapies.
Product Name:
Rat TACR2 (Tachykinin Receptor 2) CLIA Kit
SKU:
RTES00534
Target:
Rat TACR2 (Tachykinin Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TACR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TACR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TACR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TACR2. You can calculate the concentration of Rat TACR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
93-107
95-112
Average (%)
108
98
102
1:4
Range (%)
86-98
88-104
89-103
Average (%)
93
95
95
1:8
Range (%)
91-104
92-109
91-107
Average (%)
98
99
99
1:16
Range (%)
93-109
92-103
96-112
Average (%)
99
98
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.01
203.64
870.4
95.56
207.21
854.7
Standard deviation
12.57
19.71
65.54
10.62
22.63
91.45
C V (%)
12.09
9.68
7.53
11.11
10.92
10.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TACR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TACR2 in samples. No significant cross-reactivity or interference between Rat TACR2 and analogues was observed.
