The Rat Taurine Transporter (TAUT) ELISA Kit from Assay Genie is a reliable and accurate tool for the quantitative detection of TAUT levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with robust and reproducible results for their experiments.TAUT is a crucial transporter protein responsible for regulating taurine levels in cells, playing a vital role in various physiological processes including osmoregulation, membrane stabilization, and neurotransmission. Dysregulation of TAUT has been linked to conditions such as hypertension, diabetes, and neurological disorders, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
With its user-friendly protocol and high-performance features, the Rat TAUT ELISA Kit is an essential tool for researchers working in the fields of physiology, pharmacology, and neuroscience. Trust Assay Genie for your research needs and unlock the potential of TAUT in your studies. Visit www.assaygenie.com/rat-taurine-transporter-taut-elisa-kit/ to learn more and order your kit today.
Product Name:
Rat Taurine Transporter (TAUT) ELISA Kit
Product Code:
RTDL01007
Size:
96 Assays
Target:
TAUT
Synonyms:
SLC6A6, Sodium And Chloride Dependent Taurine Transporter, Solute Carrier Family 6 Member 6,Neurotransmitter Transporter,Taurine
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
