The Rat Tenascin-C (TNC) ELISA Kit is a versatile and reliable tool for measuring levels of Tenascin-C in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it ideal for a variety of research applications.Tenascin-C is an extracellular matrix glycoprotein that plays a key role in cell adhesion, migration, and tissue remodeling. It is involved in various physiological and pathological processes, including wound healing, tissue development, and cancer progression.
Detection of Tenascin-C levels can provide valuable insights into these processes and aid in the development of new therapeutic strategies.Whether studying tissue repair, cancer biology, or developmental biology, the Rat Tenascin-C ELISA Kit offers a user-friendly and reliable solution for researchers looking to quantify and analyze levels of Tenascin-C in rat samples.
Product Name:
Rat Tenascin C (TNC) ELISA Kit
Product Code:
RTDL01039
Size:
96 Assays
Target:
TNC
Synonyms:
TN-C, HXB, TN, GMEM, JI, Myotendinous antigen, Neuronectin, GP 150-225, Cytotactin, Hexabrachion, Glioma-associated-extracellular matrix antigen
Detection Method:
Sandwich
Range:
0.937-60ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
