The Rat TFF2 (Trefoil Factor 2) CLIA Kit is specifically designed for the accurate detection of TFF2 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TFF2 is a key protein that plays a role in mucosal protection and repair in the gastrointestinal tract. It is involved in promoting epithelial cell migration and proliferation, as well as protecting the mucosal lining from damage.
Dysregulation of TFF2 has been linked to gastrointestinal disorders such as ulcers, inflammatory bowel disease, and gastric cancer, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Rat TFF2 CLIA Kit provides researchers with a powerful tool for studying the role of TFF2 in gastrointestinal health and disease, offering precise and accurate measurements for a deeper understanding of its functions and potential clinical applications.
Product Name:
Rat TFF2 (Trefoil Factor 2) CLIA Kit
SKU:
RTES00572
Target:
Rat TFF2 (Trefoil Factor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TFF 2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TFF 2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TFF 2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TFF 2. You can calculate the concentration of Rat TFF 2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
95-108
93-106
Average (%)
97
100
99
1:4
Range (%)
100-117
96-107
102-115
Average (%)
107
102
107
1:8
Range (%)
102-116
93-107
98-113
Average (%)
108
98
105
1:16
Range (%)
99-115
85-99
85-101
Average (%)
105
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
99-110
105
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.71
150.17
369.85
45.84
148.62
348.08
Standard deviation
5.27
12.73
31.36
5.5
16.66
29.83
C V (%)
11.05
8.48
8.48
12.0
11.21
8.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TFF 2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TFF 2 in samples. No significant cross-reactivity or interference between Rat TFF 2 and analogues was observed.
