Rat TFPI (Tissue Factor Pathway Inhibitor) CLIA Kit
The Rat TFPI (Tissue Factor Pathway Inhibitor) CLIA Kit is a powerful tool for the quantification of TFPI levels in rat samples. This kit offers exceptional sensitivity and specificity, allowing for accurate and reproducible results in serum, plasma, and tissue culture supernatants.TFPI is a key regulator of the coagulation cascade, inhibiting the activity of tissue factor and factor VIIa. Dysregulation of TFPI has been implicated in various thrombotic disorders, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.
Whether investigating coagulation disorders, cardiovascular diseases, or other related research areas, the Rat TFPI CLIA Kit provides researchers with a reliable and efficient solution for measuring TFPI levels in rat samples.
Product Name:
Rat TFPI (Tissue Factor Pathway Inhibitor) CLIA Kit
SKU:
RTES00555
Target:
Rat TFPI (Tissue Factor Pathway Inhibitor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TFPI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TFPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TFPI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TFPI. You can calculate the concentration of Rat TFPI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
95-110
95-110
Average (%)
98
102
102
1:4
Range (%)
100-116
99-112
100-114
Average (%)
108
105
106
1:8
Range (%)
89-102
95-108
99-114
Average (%)
95
101
107
1:16
Range (%)
97-114
92-106
86-98
Average (%)
105
99
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
98
EDTA plasma (n=5)
93-106
101
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.24
134.33
453.02
56.7
142.3
485.36
Standard deviation
7.05
12.76
31.71
4.96
13.42
48.73
C V (%)
13.0
9.5
7.0
8.75
9.43
10.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TFPI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TFPI in samples. No significant cross-reactivity or interference between Rat TFPI and analogues was observed.
