Rat TFR2 (Transferrin Receptor Protein 2) CLIA Kit
The Rat TFR2 (Transferrin Receptor Protein 2) CLIA Kit is a reliable and accurate tool for the detection of transferrin receptor protein 2 levels in rat samples. This kit offers high sensitivity and specificity, providing researchers with dependable and reproducible results for a variety of research applications.Transferrin receptor protein 2 is an essential protein involved in iron metabolism, playing a key role in regulating iron uptake and storage in cells.
Dysregulation of TFR2 has been linked to various diseases, including iron overload disorders and anemia, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Rat TFR2 CLIA Kit is a valuable resource for researchers looking to investigate the role of transferrin receptor protein 2 in health and disease in rat models.
Product Name:
Rat TFR2 (Transferrin Receptor Protein 2) CLIA Kit
SKU:
RTES00564
Target:
Rat TFR2 (Transferrin Receptor Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TFR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TFR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TFR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TFR2. You can calculate the concentration of Rat TFR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
98-112
90-102
Average (%)
102
105
97
1:4
Range (%)
90-102
96-113
99-113
Average (%)
95
104
107
1:8
Range (%)
100-118
98-113
101-119
Average (%)
108
106
109
1:16
Range (%)
88-101
86-98
100-112
Average (%)
93
93
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
93
EDTA plasma (n=5)
98-110
103
Cell culture media (n=5)
98-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
213.38
435.57
1367.31
195.45
470.91
1452.89
Standard deviation
17.67
34.41
131.4
22.48
45.96
138.75
C V (%)
8.28
7.9
9.61
11.5
9.76
9.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TFR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TFR2 in samples. No significant cross-reactivity or interference between Rat TFR2 and analogues was observed.
