Rat betaTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit
The Rat βTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit is a specialized assay developed for the quantitative measurement of β-thromboglobulin (βTG), also known as platelet basic protein (PBP) or neutrophil-activating protein 2 (NAP-2), levels in a variety of rat biological samples. βTG is a chemokine and growth factor stored in the α-granules of platelets that plays a crucial role in platelet activation, chemotaxis of neutrophils, and regulating inflammatory responses. This ELISA kit provides researchers with a precise and reliable method to quantify βTG levels, enabling a deeper understanding of its involvement in platelet activation, inflammation, and cellular signaling pathways. By accurately measuring βTG concentrations, researchers can gain valuable insights into the intricate interplay between platelet function, immune responses, and inflammatory processes, shedding light on the role of βTG in various physiological and pathological conditions.
With exceptional sensitivity and specificity, the Rat βTG/PBP/CXCL7/NAP2 ELISA Kit ensures reproducible and accurate results, allowing researchers to explore the biological functions and regulatory mechanisms of βTG with precision. Manufactured following stringent quality control standards, this kit guarantees robust performance and user-friendly operation, making it an excellent choice for researchers investigating the complex roles of βTG in platelet biology, inflammation, and immune modulation.
Product Name:
Rat betaTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit
SKU:
AEES00556
Target:
Rat βTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat βTG/PBP/CXCL7/NAP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat βTG/PBP/CXCL7/NAP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat βTG/PBP/CXCL7/NAP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat βTG/PBP/CXCL7/NAP2. You can calculate the concentration of Rat βTG/PBP/CXCL7/NAP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
88-100
91-108
Average (%)
95
94
98
1:4
Range (%)
90-105
88-102
88-100
Average (%)
96
94
93
1:8
Range (%)
91-105
85-99
88-104
Average (%)
98
91
95
1:16
Range (%)
89-101
85-98
88-102
Average (%)
96
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
83-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.98
85.11
456.29
44.28
80.78
474.92
Standard deviation
2.61
5.07
19.03
2.4
4.28
18.76
C V (%)
5.55
5.96
4.17
5.42
5.3
3.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat βTG/PBP/CXCL7/NAP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat βTG/PBP/CXCL7/NAP2 in samples. No significant cross-reactivity or interference between Rat βTG/PBP/CXCL7/NAP2 and analogues was observed.
