Rat TGF- alpha (Transforming Growth Factor Alpha) CLIA Kit
The Rat TGF Alpha (Transforming Growth Factor Alpha) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of TGF Alpha levels in rat serum, plasma, and tissue culture supernatants. This kit provides reliable and reproducible results, making it an excellent choice for a variety of research applications.TGF Alpha is a key cytokine that plays a crucial role in cell growth, differentiation, and development. It is involved in various physiological processes, including wound healing, tissue regeneration, and immune response regulation.
Aberrant TGF Alpha expression has been linked to several diseases, including cancer, inflammatory disorders, and fibrotic conditions, highlighting its importance as a biomarker for disease research and therapeutic development.The Rat TGF Alpha CLIA Kit from Assay Genie is a valuable tool for studying the role of TGF Alpha in health and disease, providing researchers with accurate and reliable data to advance their scientific investigations.
Product Name:
Rat TGF- alpha (Transforming Growth Factor Alpha) CLIA Kit
SKU:
RTES00566
Target:
Rat TGF- alpha (Transforming Growth Factor Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TGF-α. You can calculate the concentration of Rat TGF-α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
85-98
87-101
Average (%)
96
92
92
1:4
Range (%)
88-101
101-120
87-97
Average (%)
95
109
92
1:8
Range (%)
85-98
97-110
92-104
Average (%)
90
104
97
1:16
Range (%)
85-97
90-105
97-111
Average (%)
92
97
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.65
57.84
242.77
25.13
58.32
265.18
Standard deviation
2.42
4.11
17.29
2.53
4.58
28.64
C V (%)
9.08
7.11
7.12
10.07
7.85
10.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TGF-α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TGF-α in samples. No significant cross-reactivity or interference between Rat TGF-α and analogues was observed.
