Rat TGF- beta2 (Transforming Growth Factor Beta 2) CLIA Kit
The Rat TGF-beta2 (Transforming Growth Factor-beta 2) CLIA Kit is a powerful tool for the quantitative measurement of TGF-beta2 levels in rat samples including serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications. TGF-beta2 is a vital cytokine involved in the regulation of cell growth, differentiation, and immune responses. It plays a crucial role in various physiological processes, including tissue repair, inflammation, and development.
Dysregulation of TGF-beta2 has been linked to numerous diseases, such as fibrosis, cancer, and autoimmune disorders, making it an important marker for studying these conditions and identifying potential therapeutic interventions. Trust the Rat TGF-beta2 CLIA Kit to provide accurate and reproducible measurements of TGF-beta2 levels, enabling researchers to gain valuable insights into the role of this cytokine in health and disease.
Product Name:
Rat TGF- beta2 (Transforming Growth Factor Beta 2) CLIA Kit
SKU:
RTES00568
Target:
Rat TGF- beta2 (Transforming Growth Factor Beta 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-β2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-β2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-β2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TGF-β2. You can calculate the concentration of Rat TGF-β2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
85-98
90-101
Average (%)
103
92
96
1:4
Range (%)
94-109
99-115
94-112
Average (%)
102
106
102
1:8
Range (%)
92-107
89-101
95-111
Average (%)
99
95
103
1:16
Range (%)
86-101
101-119
93-108
Average (%)
93
109
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.94
48.58
232.06
27.05
49.1
219.13
Standard deviation
2.46
3.51
17.89
2.62
3.76
19.13
C V (%)
9.48
7.23
7.71
9.69
7.66
8.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TGF-β2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TGF-β2 in samples. No significant cross-reactivity or interference between Rat TGF-β2 and analogues was observed.
