Rat TGF- beta2 (Transforming Growth Factor Beta 2) ELISA Kit
The Rat TGF-Beta2 (Transforming Growth Factor Beta 2) ELISA Kit is a powerful tool for accurately measuring levels of TGF-Beta2 in rat serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides precise and consistent results, making it an invaluable asset for a wide variety of research applications.TGF-Beta2 is a key cytokine involved in regulating cell growth, differentiation, and immune response. Dysregulation of TGF-Beta2 has been linked to numerous diseases, including cancer, fibrosis, and autoimmune disorders, highlighting its importance as a potential therapeutic target and biomarker.
By utilizing the Rat TGF-Beta2 ELISA Kit, researchers can gain valuable insights into the role of TGF-Beta2 in disease pathogenesis and develop innovative treatment strategies to combat these conditions. Trust in the reliability and accuracy of this kit to drive your research forward and uncover new discoveries in the field of molecular biology.
Product Name:
Rat TGF- beta2 (Transforming Growth Factor Beta 2) ELISA Kit
SKU:
RTES00848
Target:
Rat TGF- beta2 (Transforming Growth Factor Beta 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-β2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-β2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-β2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TGF-β2. You can calculate the concentration of Rat TGF-β2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
96-112
89-100
Average (%)
94
104
95
1:4
Range (%)
89-103
86-96
87-101
Average (%)
94
91
92
1:8
Range (%)
93-105
86-100
82-95
Average (%)
99
92
87
1:16
Range (%)
88-100
86-96
82-96
Average (%)
93
91
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
100
EDTA plasma (n=5)
84-99
90
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.3
122.97
358.49
45.39
123.76
338.46
Standard deviation
3.28
6.84
12.48
2.84
5.59
18.24
C V (%)
6.52
5.56
3.48
6.26
4.52
5.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TGF-β2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TGF-β2 in samples. No significant cross-reactivity or interference between Rat TGF-β2 and analogues was observed.
