Rat TGF- beta3 (Transforming Growth Factor Beta 3) ELISA Kit
The Rat TGF-beta3 (Transforming Growth Factor Beta-3) ELISA Kit is a powerful tool for the precise measurement of TGF-beta3 levels in rat serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides reliable and consistent results, making it suitable for various research applications.TGF-beta3 is a key member of the transforming growth factor beta superfamily, known for its regulatory role in cell growth, differentiation, and development. It is involved in diverse biological processes such as embryogenesis, wound healing, and immune response modulation.
Dysregulation of TGF-beta3 has been linked to various diseases including cancer, fibrosis, and autoimmune disorders, highlighting its importance as a biomarker for disease research and therapeutic development.Overall, the Rat TGF-beta3 ELISA Kit offers researchers a valuable tool for studying the role of TGF-beta3 in physiological and pathological processes, providing insights into potential therapeutic interventions for related conditions.
Product Name:
Rat TGF- beta3 (Transforming Growth Factor Beta 3) ELISA Kit
SKU:
RTES00849
Target:
Rat TGF- beta3 (Transforming Growth Factor Beta 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-β3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-β3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-β3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TGF-β3. You can calculate the concentration of Rat TGF-β3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
96-111
98-110
Average (%)
92
103
104
1:4
Range (%)
92-107
83-94
81-92
Average (%)
98
89
87
1:8
Range (%)
89-102
83-94
86-99
Average (%)
96
89
91
1:16
Range (%)
92-103
85-95
83-96
Average (%)
98
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-106
101
EDTA plasma (n=5)
96-111
101
Cell culture media (n=5)
94-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.43
157.55
414.81
43.39
168.09
436.13
Standard deviation
2.55
8.0
15.02
2.9
8.54
19.71
C V (%)
5.38
5.08
3.62
6.68
5.08
4.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TGF-β3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TGF-β3 in samples. No significant cross-reactivity or interference between Rat TGF-β3 and analogues was observed.
