Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) CLIA Kit
The Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of TIMP-1 levels in rat tissue samples. This kit provides reliable and reproducible results, making it ideal for various research applications.TIMP-1 is a key regulator of extracellular matrix remodeling and is involved in processes such as tissue repair, inflammation, and cancer progression. Elevated levels of TIMP-1 have been associated with various pathological conditions, making it a valuable biomarker for studying disease mechanisms and potential therapeutic targets.
By using the Rat TIMP-1 CLIA Kit, researchers can gain insights into the role of TIMP-1 in disease development and progression, leading to the discovery of novel diagnostic markers and therapeutic interventions. Upgrade your research capabilities with this advanced assay kit from AssayGenie.
Product Name:
Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) CLIA Kit
SKU:
RTES00323
Target:
Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TIMP-1. You can calculate the concentration of Rat TIMP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
85-98
99-117
Average (%)
103
90
108
1:4
Range (%)
88-101
88-103
100-116
Average (%)
94
95
108
1:8
Range (%)
97-109
97-113
98-112
Average (%)
102
103
104
1:16
Range (%)
96-113
91-106
96-111
Average (%)
103
97
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.33
81.76
304.61
40.19
87.62
333.62
Standard deviation
4.26
8.44
32.04
3.51
8.21
21.18
C V (%)
11.41
10.32
10.52
8.73
9.37
6.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TIMP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TIMP-1 in samples. No significant cross-reactivity or interference between Rat TIMP-1 and analogues was observed.
