Rat TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit
The Rat TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit is a specialized assay designed for the precise measurement of TIMP-2 levels in rat tissue samples. This kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for various experimental studies. TIMP-2 is a key regulator of metalloproteinases, crucial enzymes involved in extracellular matrix degradation and tissue remodeling. Dysregulation of TIMP-2 has been linked to various pathological conditions, including cancer, cardiovascular diseases, and inflammatory disorders, highlighting its potential as a valuable biomarker for disease research and therapeutic interventions.
By utilizing the Rat TIMP-2 CLIA Kit, researchers can effectively analyze TIMP-2 levels in rat tissues, providing valuable insights into the role of this protein in disease progression and therapeutic targeting. Trust in this high-quality assay kit for accurate and reliable TIMP-2 measurements in your research endeavors.
Product Name:
Rat TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit
SKU:
RTES00557
Target:
Rat TIMP-2 (Tissue Inhibitors of Metalloproteinase 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TIMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TIMP-2. You can calculate the concentration of Rat TIMP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
94-109
104-118
Average (%)
91
101
110
1:4
Range (%)
95-108
92-106
97-113
Average (%)
103
98
104
1:8
Range (%)
97-110
85-100
92-107
Average (%)
104
92
99
1:16
Range (%)
94-106
95-108
101-116
Average (%)
100
101
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
94-108
102
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
404.12
1178.28
3630.25
440.04
1135.9
3279.89
Standard deviation
36.77
85.43
220.36
49.42
118.13
266.0
C V (%)
9.1
7.25
6.07
11.23
10.4
8.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TIMP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TIMP-2 in samples. No significant cross-reactivity or interference between Rat TIMP-2 and analogues was observed.
