The Rat TLR4 (Toll-Like Receptor 4) CLIA Kit is specifically designed for the precise measurement of TLR4 levels in rat serum, plasma, and tissue homogenates. With its high sensitivity and accuracy, this kit provides dependable and consistent results, making it perfect for various research purposes.TLR4 is a key receptor in the immune response, recognizing bacterial lipopolysaccharides and initiating inflammation.
Dysregulation of TLR4 has been associated with various diseases, including sepsis, autoimmune disorders, and inflammatory conditions. Therefore, the Rat TLR4 CLIA Kit serves as a valuable tool for studying these conditions and developing potential treatments.Get your hands on the Rat TLR4 CLIA Kit today and unlock new insights into the role of TLR4 in rat physiology and pathology.
Product Name:
Rat TLR4 (Toll-Like Receptor 4) CLIA Kit
SKU:
RTES00561
Target:
Rat TLR4 (Toll-Like Receptor 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TLR4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TLR4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TLR4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TLR4. You can calculate the concentration of Rat TLR4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
91-102
92-107
Average (%)
102
96
98
1:4
Range (%)
83-96
98-113
101-114
Average (%)
90
105
106
1:8
Range (%)
94-106
89-100
101-115
Average (%)
100
94
106
1:16
Range (%)
97-111
100-116
99-112
Average (%)
103
108
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
97-109
103
Cell culture media (n=5)
101-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.69
335.65
1521.62
196.52
367.99
1446.97
Standard deviation
20.34
23.8
142.42
25.35
32.09
115.18
C V (%)
10.5
7.09
9.36
12.9
8.72
7.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TLR4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TLR4 in samples. No significant cross-reactivity or interference between Rat TLR4 and analogues was observed.
