The Rat TLR9 (Toll-Like Receptor 9) CLIA Kit is a reliable and sensitive assay designed for the accurate detection of TLR9 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit provides high specificity and reproducibility, ensuring precise and consistent results for a variety of research applications.TLR9 is an important protein involved in the immune response, specifically in recognizing and responding to bacterial DNA.
Dysregulation of TLR9 has been implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.With its superior performance and ease of use, the Rat TLR9 CLIA Kit is an essential tool for researchers looking to investigate the role of TLR9 in immune system function and inflammatory processes in rat models.
Product Name:
Rat TLR9 (Toll-Like Receptor 9) CLIA Kit
SKU:
RTES00562
Target:
Rat TLR9 (Toll-Like Receptor 9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TLR9/CD289. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TLR9/CD289 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TLR9/CD289, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TLR9/CD289. You can calculate the concentration of Rat TLR9/CD289 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-114
100-112
98-112
Average (%)
108
105
105
1:4
Range (%)
90-104
101-115
90-105
Average (%)
96
107
98
1:8
Range (%)
87-100
103-118
91-103
Average (%)
93
110
97
1:16
Range (%)
87-101
88-101
93-107
Average (%)
92
95
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
94-112
102
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.48
224.53
844.88
92.83
217.48
880.14
Standard deviation
10.64
18.23
89.47
11.98
23.9
85.2
C V (%)
10.92
8.12
10.59
12.91
10.99
9.68
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TLR9/CD289 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TLR9/CD289 in samples. No significant cross-reactivity or interference between Rat TLR9/CD289 and analogues was observed.
