The Rat TM (Thrombomodulin) CLIA Kit is a powerful tool for detecting thrombomodulin levels in rat serum, plasma, and cell culture supernatants with high accuracy. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.Thrombomodulin is a key protein involved in the regulation of blood coagulation and endothelial function, playing a critical role in various physiological processes. Dysregulation of thrombomodulin has been linked to numerous diseases, including thrombotic disorders and inflammatory conditions, making it a valuable biomarker for studying these diseases and exploring potential treatment options.
Overall, the Rat TM (Thrombomodulin) CLIA Kit is an indispensable tool for researchers looking to investigate the role of thrombomodulin in health and disease, providing reliable data for further experimentation and clinical applications.
Product Name:
Rat TM (Thrombomodulin) CLIA Kit
SKU:
RTES00546
Target:
Rat TM (Thrombomodulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TM. You can calculate the concentration of Rat TM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
93-107
97-114
Average (%)
99
101
104
1:4
Range (%)
96-107
100-115
87-99
Average (%)
101
105
93
1:8
Range (%)
89-104
103-118
93-104
Average (%)
95
109
99
1:16
Range (%)
91-103
88-100
97-110
Average (%)
96
94
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
106
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
97-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.26
112.66
456.67
48.89
110.03
411.85
Standard deviation
5.3
10.09
36.26
5.66
10.52
40.48
C V (%)
10.76
8.96
7.94
11.58
9.56
9.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TM in samples. No significant cross-reactivity or interference between Rat TM and analogues was observed.
