The Rat TNC (Tenascin-C) CLIA Kit is a highly reliable assay designed for the precise measurement of tenascin-C levels in rat serum, plasma, and tissue samples. This kit offers exceptional sensitivity and specificity, providing accurate and repeatable results for a variety of research applications.Tenascin-C is a key extracellular matrix glycoprotein that plays a crucial role in tissue development, wound healing, and cell migration. Abnormal levels of tenascin-C have been associated with various pathological conditions, such as cancer, fibrosis, and inflammatory diseases, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
Overall, the Rat TNC CLIA Kit is a valuable tool for researchers looking to investigate the role of tenascin-C in disease progression and therapeutic targeting in rat models. With its high performance and user-friendly design, this kit is ideal for advancing research in the fields of oncology, immunology, and regenerative medicine.
Product Name:
Rat TNC (Tenascin C) CLIA Kit
SKU:
RTES00540
Target:
Rat TNC (Tenascin C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TNC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TNC. You can calculate the concentration of Rat TNC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
94-108
100-111
Average (%)
97
99
105
1:4
Range (%)
100-113
99-112
93-108
Average (%)
106
106
99
1:8
Range (%)
89-103
90-101
86-100
Average (%)
95
95
92
1:16
Range (%)
91-105
96-109
85-100
Average (%)
97
103
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-98
93
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
99-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.63
113.83
392.41
38.24
103.1
397.76
Standard deviation
3.43
8.28
37.95
4.72
8.38
39.5
C V (%)
8.66
7.27
9.67
12.34
8.13
9.93
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TNC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TNC in samples. No significant cross-reactivity or interference between Rat TNC and analogues was observed.
