The Rat TNC (Tenascin-C) ELISA Kit is specifically developed for the precise measurement of tenascin-C levels in rat serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides reliable and consistent results, making it perfect for various research purposes.Tenascin-C is a vital extracellular matrix protein known for its role in cell adhesion, migration, and tissue remodeling.
Its expression is associated with numerous pathological conditions including cancer, fibrosis, and inflammation. By accurately quantifying tenascin-C levels, researchers can gain valuable insights into disease processes and potential therapeutic targets.Don't miss out on this invaluable tool for your research. Order the Rat TNC (Tenascin-C) ELISA Kit now and unlock the secrets of tenascin-C biology in rat models.
Product Name:
Rat TNC (Tenascin C) ELISA Kit
SKU:
RTES00789
Target:
Rat TNC (Tenascin C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNC. You can calculate the concentration of Rat TNC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
92-109
97-108
Average (%)
100
99
102
1:4
Range (%)
89-103
86-98
82-98
Average (%)
94
92
89
1:8
Range (%)
88-101
82-94
83-94
Average (%)
95
86
88
1:16
Range (%)
93-107
84-98
89-103
Average (%)
98
91
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
97
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
88-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.28
232.1
731.9
118.77
225.68
795.29
Standard deviation
6.45
11.37
29.72
7.63
9.34
40.88
C V (%)
5.96
4.9
4.06
6.42
4.14
5.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TNC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TNC in samples. No significant cross-reactivity or interference between Rat TNC and analogues was observed.
