Rat TNF- beta (Tumor Necrosis Factor beta) CLIA Kit
The Rat TNF-beta (Tumor Necrosis Factor-beta) CLIA Kit is a highly sensitive assay designed to accurately detect TNF-beta levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional specificity, providing reliable and consistent results for various research applications.TNF-beta is a key cytokine involved in inflammation and immune response, playing a critical role in various physiological and pathological processes. Its dysregulation is associated with inflammatory diseases, autoimmune disorders, and cancer, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic interventions.
With its advanced technology and robust performance, the Rat TNF-beta CLIA Kit is an indispensable tool for researchers studying the role of TNF-beta in health and disease. Get reliable and precise results with this innovative assay kit from Assay Genie.
Product Name:
Rat TNF- beta (Tumor Necrosis Factor beta) CLIA Kit
SKU:
RTES00579
Target:
Rat TNF- beta (Tumor Necrosis Factor beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TNF-β. You can calculate the concentration of Rat TNF-β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
100-114
92-106
Average (%)
96
106
100
1:4
Range (%)
90-103
97-111
102-119
Average (%)
96
103
109
1:8
Range (%)
88-102
84-97
88-100
Average (%)
94
90
93
1:16
Range (%)
101-119
92-103
100-113
Average (%)
109
98
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.61
82.58
321.58
43.43
78.34
305.1
Standard deviation
3.45
7.91
28.52
5.06
7.54
23.34
C V (%)
8.1
9.58
8.87
11.65
9.62
7.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TNF-β concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TNF-β in samples. No significant cross-reactivity or interference between Rat TNF-β and analogues was observed.
