Rat TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) CLIA Kit
The Rat TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily Member 1B) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of TNFRSF1B levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.TNFRSF1B is a critical cell surface receptor involved in mediating the effects of tumor necrosis factor-alpha (TNF-α), a key cytokine involved in inflammation, cell survival, and apoptosis.
Dysregulation of TNFRSF1B has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and cancer.By accurately quantifying TNFRSF1B levels, researchers can gain valuable insights into the role of this receptor in disease pathogenesis, potentially leading to the development of novel therapeutic strategies. This CLIA kit offers a convenient and efficient method for studying TNFRSF1B biology in rat models, enabling advancements in biomedical research.
Product Name:
Rat TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) CLIA Kit
SKU:
RTES00578
Target:
Rat TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TNFRSF1B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNFRSF1B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNFRSF1B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TNFRSF1B. You can calculate the concentration of Rat TNFRSF1B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
87-99
92-105
Average (%)
100
92
98
1:4
Range (%)
87-99
88-98
100-116
Average (%)
94
93
106
1:8
Range (%)
98-114
95-110
95-110
Average (%)
105
101
103
1:16
Range (%)
95-110
98-110
83-99
Average (%)
101
104
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-102
97
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
98-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.61
68.68
247.4
29.53
66.11
233.13
Standard deviation
2.92
5.49
25.88
3.06
6.61
16.34
C V (%)
10.58
7.99
10.46
10.36
10.0
7.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TNFRSF1B concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TNFRSF1B in samples. No significant cross-reactivity or interference between Rat TNFRSF1B and analogues was observed.
